Dynamics of corneal endothelial cell death in organ culture
نویسندگان
چکیده
منابع مشابه
Comparative analysis of proteoglycans synthesized by chick corneal stromal cells in cell culture and organ culture.
Proteoglycans (PGs) synthesized by chick corneal stromal cells in cell culture and organ culture were metabolically radiolabelled with [35S]sulfate (for glycosaminoglycans) and [3H]leucine (for core proteins). Media, cell extracts and organ extracts from cultures were chromatographed on DEAE-Sephacel columns and separated into three fractions: the pass-through fraction (Fraction 1: nonsulfated ...
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PURPOSE To evaluate the effect of media composition and storage method on pre-prepared Descemet's membrane endothelial keratoplasty (DMEK) grafts. METHODS 50 corneas were used. Endothelial wound healing and proliferation in different media were assessed using a standard injury model. DMEK grafts were stored using three methods: peeling with free scroll storage; partial peeling with storage on...
متن کاملCorneal glycosaminoglycan synthesis in long-term organ culture.
Rabbit corneas cultured in a serum-free, dextran-supplemented medium maintained a high rate of incorporation of H-glucosamine into glycosaminoglycans (GAG) for several weeks in culture. The labeled GAG secreted by cultured corneas was identified as keratan sulfate, galactosaminoglycan, and heparan sulfate. Corneal GAG labeled in vivo consisted primarily of keratan sulfate and galactosaminoglyca...
متن کاملNeutral glycolipids of migrating and nonmigrating rabbit corneal epithelium in organ and cell culture.
It is generally believed that plasma membrane glycoconjugates influence corneal epithelial cell migration after wounding. Previous studies have focused on the role of glycoproteins in this event. The present study was designed to determine whether migration-specific glycolipids are synthesized by epithelium of healing rabbit corneas. Migrating and nonmigrating rabbit corneal epithelia were incu...
متن کاملGangliosides of migrating and nonmigrating corneal epithelium in organ and cell culture.
PURPOSE To identify major gangliosides - the sialated glycolipids - of corneal epithelium; to determine which specific gangliosides, if any, are synthesized in a higher amount or are downregulated during corneal epithelial cell migration; and to determine what role, if any, they play in the modulation of corneal epithelial cell proliferation. METHODS [3H]-galactose-labeled and unlabeled glyco...
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ژورنال
عنوان ژورنال: British Journal of Ophthalmology
سال: 2003
ISSN: 0007-1161
DOI: 10.1136/bjo.87.3.376-b